Immunostaining:Article Title: Endothelial AGGF1 promotes retinal angiogenesis by coordinating TNFSF12/FN14 signalling
Article Snippet: Intravitreal injections:anti-IgG (1:50, Cell Signaling Technology, 2729s), anti-AGGF1 (1:50, Proteintech, 11889-1-AP), anti-TNFSF12 (1:50, Proteintech, 12537-1-AP) or anti-VEGF (1:50, Proteintech, 19003-1-AP). .. Immunostaining of whole-mount retinas:anti-AGGF1 (1:200, Novus, NB100-455), anti-GFAP (1:200, Proteintech, 60190-1-Ig), antiIba-1 (1:200, Abcam, ab283319), anti-TER119 (1:100, Invitrogen, 14-5921-81) and anti-F4/80 (1:200, Proteintech, 28463-1-AP). .. Co-Immunoprecipitation (Co-IP): anti-AGGF1 (1:100, Proteintech, 11889-1-AP), anti-TNFSF12 (1:100, Novus, NBP1-97624), and antiFN14 (1:50, Zenbio, R381352) All commercially available primary antibodies have been validated by manufactures: 4 nature portfolio | rep orting sum m ary April2023 Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Wild animals Reporting on sex Field-collected samples Ethics oversight Note that full information on the approval of the study protocol must also be provided in the manuscript. anti-AGGF1 : https://www.ptgcn.com/products/AGGF1-Antibody-11889-1-AP.htm anti-AGGF1:https://www.novusbio.com/products/vg5q-antibody_nb100-455 anti-VEGF :https://www.ptgcn.com/products/VEGFA-Antibody-19003-1-AP.htm anti-TNFSF12:https://www.ptgcn.com/products/TNFSF12-Antibody-12537-1-AP.htm anti-TNFSF12:https://www.novusbio.com/products/tweak-tnfsf12-antibody-mira-2_nbp1-97624 anti-FN14 :https://abclonal.com.cn/catalog/A5955 anti-FN14:http://www.zen-bio.cn/prod_view.aspx?TypeId=245&Id=662418&FId=t3:245:3 anti-CyclinA2:https://www.ptgcn.com/products/CCNA2-Antibody-18202-1-AP.htm anti-CyclinD1:https://www.ptgcn.com/products/CCND1-Antibody-60186-1-Ig.htm anti-CDK1:https://www.ptgcn.com/products/CDC2-Specific-Antibody-19532-1-AP.htm anti- HIF-1:https://www.ptgcn.com/products/HIF1A-Antibody-20960-1-AP.htm anti--Tubulin:https://www.bioworlde.com/index.php?c=product&a=search&keyword=AP0064 anti-CD31:https://www.ptgcn.com/products/CD31-Antibody-66065-2-Ig.htm anti-CD31:https://www.ptgcn.com/products/PECAM1-Antibody-11265-1-AP.htm anti-IgG:https://www.cellsignal.cn/products/primary-antibodies/normal-rabbit-igg/2729 anti-GFAP:https://www.ptgcn.com/products/GFAP-Antibody-60190-1-Ig.htm anti-Iba-1:https://www.abcam.cn/products/primary-antibodies/iba1-antibody-epr16589-mouse-igg1-chimeric-ab283319.html anti-F4/80:https://www.ptgcn.com/products/F4-80-Antibody-28463-1-AP.htm anti-TER119:https://www.thermofisher.cn/cn/zh/antibody/product/TER-119-Antibody-clone-TER-119-Monoclonal/14-5921-81 Human retinal microvascular endothelial cells (HRMECs) were acquired from BNCC (BeNa Culture Collection).
|
Novus Biologicals is a verified supplier 
Novus Biologicals manufactures this product 